Active Recombinant Human IL12RB2 Protein, Fc/His-tagged

• Cat.No.: IL12RB2-9483H
• Product Overview: Recombinant Human IL-12 R beta 2 protein (Lys24-Asn622) with Human IgG1 (Pro100-Lys330) and a 6-His tag at C-terminus was expressed in mouse myeloma cell line.

Specification

• Description: The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: His&Fc
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑12 is immobilized at 0.5 µg/mL, 100 µL/well, the concentration of Recombinant Human IL‑12 R beta 2 Fc that produces 50% of the optimal binding response is 0.015-0.09 µg/mL.
• Molecular Mass: 95 kDa
• N-terminal Sequence Analysis: Lys24
• Endotoxin: <0.10 EU per 1 µg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 250 µg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.
• Protein length: Lys24-Asn622
• Publication:
  Human IL-35 Inhibits the Bioactivity of IL-12 and Its Interaction with IL-12Rβ2 (2023)

Gene Information

• Gene Name: IL12RB2 interleukin 12 receptor subunit beta 2 [ Homo sapiens (human) ]
• Official Symbol: IL12RB2
• Synonyms: IL12RB2; interleukin 12 receptor subunit beta 2; interleukin-12 receptor subunit beta-2; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain
• Gene ID: 3595
• mRNA Refseq: NM_001559
• Protein Refseq: NP_001550
• MIM: 601642
• UniProt ID: Q99665

Reviews

08/14/2022

Good for receptor binding studies.

03/17/2022

Consistent in bioactivity.

07/31/2017

Suitable for flow cytometry.

Q&As

Could you explain the benefits of using CRISPR/Cas9-mediated gene tagging to track IL12RB2 localization?

CRISPR/Cas9 gene tagging tracks IL12RB2 localization dynamically.

What methods are suitable for assessing IL12RB2 dimerization and its effect on downstream signaling?

Crosslinking and immunoprecipitation study IL12RB2 dimerization.

How can ChIP-seq analysis provide insights into the genes regulated by IL12RB2?

ChIP-seq identifies genes under IL12RB2's regulation.

How does quantitative RT-PCR validate IL12RB2's impact on cytokine-induced gene expression?

Quantitative RT-PCR validates IL12RB2's impact on gene expression.

What experimental approaches can validate IL12RB2's involvement in signal transduction pathways?

Functional assays validate IL12RB2's role in signaling pathways.

What are the advantages of utilizing flow cytometry to analyze cell-specific IL12RB2 expression?

Flow cytometry quantifies IL12RB2 expression on specific cell types.

Can you elaborate on the use of confocal microscopy to visualize the subcellular distribution of IL12RB2?

Confocal microscopy visualizes IL12RB2 distribution in cells.

How can siRNA knockdown experiments reveal the functional role of IL12RB2?

siRNA knockdown reveals IL12RB2's functional significance.

How does co-immunoprecipitation assist in understanding the protein-protein interactions of IL12RB2?

Co-immunoprecipitation unveils IL12RB2's protein interactions.

What techniques are effective in studying IL12RB2's interaction with cytokines IL12A and IL23A?

Co-immunoprecipitation and ELISA assay cytokine interactions with IL12RB2.