Recombinant Mouse Il12rb2 protein, His-tagged

• Cat.No.: Il12rb2-1466M
• Product Overview: Recombinant Mouse Il12rb2 aa. (Met285~Gln441 (Accession # P97378)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 19.3kDa.
• Protein length: Met285~Gln441 (Accession # P97378)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 8.7.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Il12rb2 interleukin 12 receptor, beta 2 [ Mus musculus (house mouse) ]
• Official Symbol: Il12rb2
• Synonyms: Ifnm; IL-12RB2; A930027I18Rik; IL-12 receptor subunit beta-2; IL-12R subunit beta-2; interferon response to microorganisms; interleukin-12 receptor subunit beta-2
• Gene ID: 16162
• mRNA Refseq: NM_001311141.1
• Protein Refseq: NP_001298070.1
• UniProt ID: P97378

Reviews

08/14/2022

Good for receptor binding studies.

03/17/2022

Consistent in bioactivity.

07/31/2017

Suitable for flow cytometry.

Q&As

Could you explain the benefits of using CRISPR/Cas9-mediated gene tagging to track IL12RB2 localization?

CRISPR/Cas9 gene tagging tracks IL12RB2 localization dynamically.

What methods are suitable for assessing IL12RB2 dimerization and its effect on downstream signaling?

Crosslinking and immunoprecipitation study IL12RB2 dimerization.

How can ChIP-seq analysis provide insights into the genes regulated by IL12RB2?

ChIP-seq identifies genes under IL12RB2's regulation.

How does quantitative RT-PCR validate IL12RB2's impact on cytokine-induced gene expression?

Quantitative RT-PCR validates IL12RB2's impact on gene expression.

What experimental approaches can validate IL12RB2's involvement in signal transduction pathways?

Functional assays validate IL12RB2's role in signaling pathways.

What are the advantages of utilizing flow cytometry to analyze cell-specific IL12RB2 expression?

Flow cytometry quantifies IL12RB2 expression on specific cell types.

Can you elaborate on the use of confocal microscopy to visualize the subcellular distribution of IL12RB2?

Confocal microscopy visualizes IL12RB2 distribution in cells.

How can siRNA knockdown experiments reveal the functional role of IL12RB2?

siRNA knockdown reveals IL12RB2's functional significance.

How does co-immunoprecipitation assist in understanding the protein-protein interactions of IL12RB2?

Co-immunoprecipitation unveils IL12RB2's protein interactions.

What techniques are effective in studying IL12RB2's interaction with cytokines IL12A and IL23A?

Co-immunoprecipitation and ELISA assay cytokine interactions with IL12RB2.