Active Recombinant Human IL13
|Product Overview :||Recombinant Human IL13 (AAK53823.1) (Met 1-Asn 132) was produced in CHO Cells.|
- Gene Information
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|Form :||Lyophilized from sterile 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
|Bio-activity :||Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/mL.|
|Molecular Mass :||The recombinant human IL13 consists of 112 amino acids and migrates as an approximately 12.5 kDa band in SDS-PAGE under reducing conditions as predicted.|
|Protein Length :||Met 1-Asn 132|
|Endotoxin :||< 1.0 EU per μg protein as determined by the LAL method.|
|Purity :||> 92 % as determined by SDS-PAGE|
|Storage :||Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 150°C before opening to recover the entire contents.|
|Gene Name :||IL13 interleukin 13 [ Homo sapiens ]|
|Official Symbol :||IL13|
|Synonyms :||IL13; interleukin 13; interleukin-13; allergic rhinitis; ALRH; BHR1; Bronchial hyperresponsiveness 1 (bronchial asthma); IL 13; MGC116786; MGC116788; MGC116789; P600; Bronchial hyperresponsiveness-1 (bronchial asthma); IL-13;|
|Gene ID :||3596|
|mRNA Refseq :||NM_002188|
|Protein Refseq :||NP_002179|
|UniProt ID :||P35225|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Flow cytometry quantifies IL13 receptor expression on cells.
Transgenic models illuminate IL13's in vivo functions.
ELISA quantifies IL13 secretion in cell cultures accurately.
siRNA knockdown uncovers IL13's specific cellular effects.
Co-immunoprecipitation identifies IL13 signaling partners.
Differential techniques distinguish IL13 and IL4 signaling pathways.
Reporter gene assays gauge IL13-driven transcription.
Microarray analysis profiles IL13-induced gene expression.
Experimental methods reveal IL13's impact on tissue remodeling.
Site-directed mutagenesis identifies key residues in IL13 receptor binding.
Customer Reviews (3)Write a review
Effective in Th2 cell differentiation. -
Good for cytokine assays. -
High yield in expression. -
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