Active Recombinant Human IL1B protein, R-PE labeled

• Cat.No.: IL1B-02HP
• Product Overview: Recombinant Human IL1B protein was expressed in Escherichia coli. Purified protein was labeled with R-PE in vitro.

Specification

• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
• Source: E. coli
• Species: Human
• Form: Solution
• Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is less or equal 12pg/ml.
• Molecular Mass: Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids.
• Protein Length: 154 aa
• AA Sequence: MAPVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
• Endotoxin: Less than 1 EU/µg of rHuIL-1β as determined by LAL method.
• Purity: >95% by SDS-PAGE
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Concentration: 1.0 mg/ml

Gene Information

• Gene Name: IL1B interleukin 1 beta [ Homo sapiens (human) ]
• Official Symbol: IL1B
• Synonyms: IL-1; IL1F2; IL1beta; IL1-BETA
• Gene ID: 3553
• mRNA Refseq: NM_000576.3
• Protein Refseq: NP_000567.1
• MIM: 147720
• UniProt ID: P01584