Active Recombinant Human IL2 (21-153aa), Met tagged

• Cat.No.: IL2-028E
• Product Overview: Recombinant Human IL2 (Ala21 ­ Thr153, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Interleukin­2 (IL­2) is a O­glycosylated, four μ­helix bundle cytokine that has potent stimulatory activity for antigen­activated T cells. It is expressed by CD4+ and CD8+ T cells, μ? T cells, B cells, dendritic cells, and eosinophils. IL­2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL­2 and IL­2 Rμ synthesis. It contributes to T cell homeostasis by promoting the Fas­induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes. IL­2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL­2 may be a key cytokine in the natural suppression of autoimmunity.
• N­terminal Sequence Analysis: Met
• Molecular Mass: 15 kDa
• Activity: Measured in a cell proliferation assay using CTLL­2 mouse cytotoxic T cells. The ED50 for this effect is typically 0.075 ­ 0.375 ng/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 200 μg/mL in sterile 10 mM Sodium Acetate, pH 4.0.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 3 month, 2 to 8 °C under sterile conditions after reconstitution.
• Unitprot ID: P60568

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; IL-2; TCGF; lymphokine; interleukin-2; aldesleukin; T cell growth factor; involved in regulation of T-cell clonal expansion
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• Chromosome Location: 4q26-q27
• Pathway: Calcium signaling in the CD4+ TCR pathway; Cytokine Signaling in Immune system; Downstream signaling in naive CD8+ T cells; IL-2 Signaling Pathway; Inflammatory Response Pathway; Jak-STAT signaling pathway; T cell receptor signaling pathway
• Function: carbohydrate binding; cytokine activity; glycosphingolipid binding; growth factor activity; interleukin-2 receptor binding; kappa-type opioid receptor binding; kinase activator activity