Active Recombinant Human IL2

• Cat.No.: IL2-341H
• Product Overview: Human IL2 (NP_000577, 22 a.a.-153 a.a.) partial recombinant protein expressed in Escherichia coli.

Specification

• Cat. No.: IL2-341H
• Description: Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body"s natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity.
• Sequence: MPTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFSQSIIS TLT
• Theoretical MW (kDa): 15.4
• Form: Liquid
• Preparation Method: Escherichia coli expression system
• Purity: > 95% by SDS-PAGE
• Activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 1.5 ng/mL.
• Application: SDS-PAGE
• Storage Buffer: In PBS, pH 7.4
• Storage: Store at 4°C for 1-2 weeks. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing.
• Unitprot ID: P60568
• Pathways: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; T cell receptor signaling pathway; Type I diabetes mellitus
• Tag: Non
• Publication:
  Cytokine and reactivity profiles in SLE patients following anti-CD19 CART therapy (2023)

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL-2; TCGF; lymphokine; T-cell growth factor; Aldesleukin, Interleukin-2; interleukin-2 precursor; involved in regulation of T-cell clonal expansion; IL2; interleukin 2
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• Chromosome Location: 4q26-q27
• Function: interleukin-2 receptor binding; kinase activator activity

Reviews

04/13/2022

Potent in T-cell activation.

07/27/2018

Suitable for cytokine assays.

01/07/2018

High stability.

Q&As

What is the impact of IL2 on T-cell memory formation?

IL2 promotes the formation of memory T-cells.

What is the role of IL2 in the tumor microenvironment?

IL2 has a pro-tumorigenic role in the tumor microenvironment.

How does IL2 interact with other cytokines in a mixed environment?

IL2 synergizes with IL-12 in a mixed cytokine environment.

Does IL2 modulate the expression of checkpoint inhibitors?

IL2 upregulates the expression of PD-1 checkpoint inhibitors.

What are the kinetics of IL2 internalization in T cells?

IL2 is rapidly internalized by activated T-cells.

Does IL2 influence the polarization of macrophages?

IL2 induces M1 macrophage polarization.

How does IL2 affect the blood-brain barrier permeability?

IL2 increases blood-brain barrier permeability.

How does IL2's affinity for its receptor change under hypoxic conditions?

IL2's affinity for its receptor decreases under hypoxic conditions.

What post-translational modifications affect IL2's bioactivity?

IL2's bioactivity is enhanced by glycosylation.

What is the role of IL2 in the differentiation of Th17 cells?

IL2 promotes the differentiation of Th17 cells.