Active Recombinant Human IL2 protein

• Cat.No.: IL2-502H
• Product Overview: Recombinant Human IL2 protein(NP_000577.2)(Ala21-Thr153) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: Ala21-Thr153
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1.Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-2.5 ng/mL.2.Immobilized CD25/IL2RA Protein,Human,Recombinant (ECD,hFc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Human IL2 Protein,Low Endotoxin, The EC50 is 2-8 ng/mL.
• Molecular Mass: The recombinant human IL2 consists of 133 amino acids and predicts a molecular mass of 15.4 kDa. It migrates as an approximately 18.52 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 0.01 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
  > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; IL 2; T cell growth factor; TCGF; aldesleukin; involved in regulation of T-cell clonal expansion; IL-2; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586
• Protein Refseq: NP_000577
• MIM: 147680
• UniProt ID: P60568