Active Recombinant Human IL23A&Mouse IL12B protein, His-Avi-tagged

• Cat.No.: IL23A& IL12B-1342H
• Product Overview: Active Recombinant Human IL23A&Mouse IL12B protein(Q9NPF7&P43432-1)(Arg20-Pro189&Met23-Ser335) is expressed from HEK293 with His tag and Avi tag at the C-Terminus of IL-23 alpha.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 20-189 aa&23-335 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human IL-23 alpha&Mouse IL-12 beta, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-23R, mFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta). Due to glycosylation, the protein migrates to 22 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ]
• Official Symbol: Il23A
• Synonyms: IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL 23; IL 23A; IL23P19; interleukin six; G CSF related factor; P19; SGRF; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor; IL-23; IL-23A; MGC79388
• Gene ID: 51561
• mRNA Refseq: NM_016584
• Protein Refseq: NP_057668
• MIM: 605580
• UniProt ID: Q9NPF7
• Gene Name 2: Il12b interleukin 12b [ Mus musculus ]
• Official Symbol 2: Il12B
• Synonyms 2: IL12B; interleukin 12b; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; p40; Il-12b; Il12p40; Il-12p40
• Gene ID 2: 16160
• mRNA Refseq 2: NM_008352
• Protein Refseq 2: NP_032378