Active Recombinant Human IL2RA, His tagged

• Cat.No.: IL2RA-637H
• Product Overview: Recombinant Human IL2RA extracellular domain (NP_000408.1) (Met 1-Cys 213), fused with a C-terminal polyhistidine tag, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Cys213
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: Measured by its ability to inhibit IL2-induced proliferation of M07e cells. The ED50 for this effect is 0.2-1.2 μg/mL in the presence of 30 ng/mL of recombinant human IL2.
• Molecular Mass: The recombinant human IL2Rα consists of 203 amino acids and has a predicted molecular mass of 23.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL2Rα is approximately 44 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL2RA interleukin 2 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL2RA
• Synonyms: IL2RA; interleukin 2 receptor, alpha; IL2R; interleukin-2 receptor subunit alpha; CD25; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha; TCGFR; IDDM10
• Gene ID: 3559
• mRNA Refseq: NM_000417
• Protein Refseq: NP_000408
• MIM: 147730
• UniProt ID: P01589