Active Recombinant Human IL4R protein, Fc/Avi-tagged, Biotinylated

• Cat.No.: IL4R-051H
• Product Overview: Biotinylated Recombinant Human IL4R(Gly24-His232) protein, fused to Fc/Avi tag at the C-terminus, was expressed in HEK293 cells .

Specification

• Description: Interleukin 4 Receptor alpha (IL-4R alpha ), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family. IL-4R alpha plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis (1-5). Mature human IL-4R alpha consists of a 207 amino acid (aa) extracellular domain (ECD) that contains a cytokine binding region and one fibronectin type III domain, a 24 aa transmembrane segment, and a 569 aa cytoplasmic domain that contains one Box 1 motif and one ITIM motif (6, 7). Within the ECD, human IL-4R alpha shares 51% aa sequence identity with mouse and rat IL-4R alpha. Soluble forms of IL-4R alpha, generated by alternate splicing or proteolysis, retain ligand binding properties and inhibit IL-4 bioactivity (8-11). IL-4R alpha is a component of two distinct receptor complexes and shows species selectivity between human and mouse (6). It can associate with the common gamma chain ( gamma c) to form the IL-4 responsive type I receptor in which gamma c increases the affinity for IL-4 and enables signaling (12, 13). It can alternatively associate with IL-13R alpha 1 to form the type II receptor which is responsive to both IL-4 and IL-13 (14, 15). The use of shared receptor components contributes to the overlapping biological effects of IL-4 and IL-13 as well as other cytokines that utilize gamma c (i.e. IL-2, IL-7, IL-9, IL-15, and IL-21) (16, 17).
• Source: HEK293 cells
• Species: Human
• Tag: Fc&Avi
• Predicted N Terminal: Gly24
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its ability to inhibit IL4-dependent proliferation of TF1 human erythroleukemic cells.The ED50 for this effect is 5-30 ng/mL in the presence 0.2 ng/mL of Recombinant Human IL4. When Biotinylated Recombinant Human IL4R Fc Chimera Avi-tag is captured , the concentration of Recombinant Human IL4 that produces 50% of the optimal binding response is approximately 3-20 ng/mL.
• Molecular Mass: 73-82 kDa, under reducing conditions
• Protein length: Gly24-His232
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Binding Activity, Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 250 μg/mL in PBS.

Gene Information

• Gene Name: IL4R interleukin 4 receptor [ Homo sapiens ]
• Official Symbol: IL4R
• Synonyms: IL4R; interleukin 4 receptor; interleukin-4 receptor subunit alpha; CD124; IL-4 receptor subunit alpha; interleukin-4 receptor alpha chain; IL4RA; IL-4RA
• Gene ID: 3566
• mRNA Refseq: NM_000418
• Protein Refseq: NP_000409
• MIM: 147781
• UniProt ID: P24394

Reviews

06/30/2022

Easy to attach to the surface of cells or materials.

09/29/2021

It has growth factor activity to promote cell growth and proliferation.

02/27/2019

In animals or humans caused by the prone production of antibodies.

Q&As

How about function of IL4R?

IL4R is a synthetic protein that mimics the IL-4 signaling pathway in nature and binds to the IL-4 receptor in cells, which can be used to study the IL-4 signaling pathway and related diseases.

What is the production technology of IL4R?

Common IL4R production technologies include prokaryotic expression systems and mammalian cell expression systems.

Which application of IL4R in tumor therapy?

This protein is used in tumor immunotherapy, which can promote anti-tumor immune response and enhance the anti-tumor effect of cytotoxic T lymphocytes.

In what fields does IL4R have applications?

It is mainly used in immunology, drug research and other fields. It is also widely used in vaccines, biotechnology, cancer treatment and so on.

Are there have application of IL4R in the treatment of asthma?

IL4R is used to treat severe pheochromocytome-induced aerodoritis and was approved by the FDA as the first drug to treat this disease.

Can IL4R be administered orally?

IL4R cannot generally be administered orally, and needs to be administered by injection or intravenous drip.