| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Fc |
| Protein Length : |
26-232 a.a. |
| Form : |
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Immobilized Human IL-4, Tag Free at 5 μg/mL (100 μL/well)can bind Human IL-4 R alpha, Fc Tag with a linear range of 1-20 ng/mL.Immobilized Human IL-4 R alpha, Fc Tag at 5 μg/mL (100 μL/well)can bind Biotinylated Human IL-4, Avitag,His Tag with a linear range of 0.1-2 ng/mL.Serial dilutions of Monoclonal IL-4R/CD124 Neutralizing Antibody were added into Human IL-4 R alpha, Fc Tag: Biotinylated Human IL-4, Avitag,His Tag binding reactions. The half maximal inhibitory concentration (IC50) is 1.144 μg/mL. |
| Molecular Mass : |
This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 50.4 kDa. The protein migrates as 57-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Proteinlength : |
Met26-His232 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
