Active Recombinant Human IL6R protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL6R-0185H
• Product Overview: Biotinylated Recombinant Human IL6R protein(Leu20-Pro365), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 20-365 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human IL-6, premium grade at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-6 R alpha, Fc,Avitag with a linear range of 0.3-20 ng/mL.Immobilized Human gp130, His Tag can bind Biotinylated Human IL-6 R alpha, Fc,Avitag with a linear range of 0.6-20 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag)The protein has a calculated MW of 66.7 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu20-Pro365
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: IL6R interleukin 6 receptor [ Homo sapiens ]
• Official Symbol: IL6R
• Synonyms: IL6R; interleukin 6 receptor; interleukin-6 receptor subunit alpha; CD126; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha; gp80; IL6RA; IL-6RA; IL-6R-1; MGC104991
• Gene ID: 3570
• mRNA Refseq: NM_000565
• Protein Refseq: NP_000556
• MIM: 147880
• UniProt ID: P08887