Active Recombinant Human INSR Protein, His & GST-tagged

• Cat.No.: INSR-294H
• Product Overview: Recombinant human INSR isoform long (NP_000199.2) cytoplasmic domain (Gly 989-Ser 1382) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 989-1382 a.a.
• Predicted N Terminal: Met
• Form: Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4, 20% gly, 0.3mM DTT.
• Bio-activity: The specific activity was determined to be 45 nmol/min/mg using Poly(Ala,Glu,Lys,Tyr)6:2:5:1 as substrate.
• Molecular Mass: The recombinant human INSR (989-1382)/GST chimera consists of 631 amino acids and has a calculated molecular mass of 72.3 KDa. It migrates as an approximately 70 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: INSR insulin receptor [ Homo sapiens ]
• Official Symbol: INSR
• Synonyms: INSR; insulin receptor; CD220; IR; HHF5
• Gene ID: 3643
• mRNA Refseq: NM_000208
• Protein Refseq: NP_000199
• MIM: 147670
• UniProt ID: P06213
• Chromosome Location: 19p13.3-p13.2
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; IRS activation, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem