Active Recombinant Human Insulin-like Growth Factor 1

• Cat.No.: IGF1-658H
• Product Overview: IGF1 Des(1-3) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids (aa 4-70) and having a molecular mass of 7372 Dalton. IGF-1 Des1-3 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: IGF1-658H
• Description: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed "sulfation factor," which later became known as "somatomedin". Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190).
• Source: Human
• Host: Escherichia Coli.
• Form: The protein was lyophilized after extensive dialysis against 50mM acetic acid buffer.
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Bio-activity: The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1x106 U/mg. For most in-vitro applications, IGF1 des1-3 exerts its biological activity in the concentration range of 0.2-20 ng/ml.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized IGF1 Des(1-3) in 100mM Acetic acid not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Leu-Cys-Gly-Ala.
• Storage: Lyophilized IGF1 des(1-3) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 des-1-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IGF1 insulin-like growth factor 1 (somatomedin C) [ Homo sapiens ]
• Official Symbol: IGF1
• Synonyms: IBP1; IGF-I; IGF-IA; IGFI; MGF; Somatomedin-C; Mechano growth factor; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); somatomedin C; IGF1B_HUMAN; Insulin-like growth factor IB [Precursor]; IGF-IB.
• Gene ID: 3479
• mRNA Refseq: NM_000618
• Protein Refseq: NP_000609
• MIM: 608747
• UniProt ID: P01343
• Chromosome Location: 12q23.2
• Pathway: Focal adhesion; Glioma; Long-term depression; Melanoma; Prostate cancer; mTOR signaling pathway; Hemostasis
• Function: Growth factor activity; Hormone activity; Hormone activity; Insulin receptor binding; Insulin-like growth factor receptor binding; Protein binding