Active Recombinant Human ITGA1&ITGB1 protein, His-tagged

• Cat.No.: ITGA1&ITGB1-0311H
• Product Overview: Recombinant Human ITGA1&ITGB1 protein(Phe29-Pro1141(ITGA1)&Gln21-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 1-20 ng/mL.
• Molecular Mass: The protein migrates as 170-200 kDa (ITGA1) and 100-130 kDa (ITGB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe29-Pro1141(ITGA1)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA1 integrin, alpha 1 [ Homo sapiens ]
• Official Symbol: ITGA1
• Synonyms: ITGA1; integrin, alpha 1; integrin alpha-1; CD49a; VLA1; VLA-1; laminin and collagen receptor; very late activation protein 1; CD49 antigen-like family member A
• Gene ID: 3672
• mRNA Refseq: NM_181501
• Protein Refseq: NP_852478
• MIM: 192968
• UniProt ID: P56199