Active Recombinant Rat ITGA1&ITGB1 protein, His-tagged

• Cat.No.: ITGA1&ITGB1-0309R
• Product Overview: Recombinant Rat ITGA1&ITGB1 protein(Phe29-Pro1142(ITGA1)&Gln21-Asp729(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.02-0.313 μg/mL.Immobilized Human Collagen IV Protein at 2 μg/mL (100 μL/well) can bind Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.002-0.156 μg/mL.
• Molecular Mass: Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
• Proteinlength: Phe29-Pro1142(ITGA1)&Gln21-Asp729(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Itga1 integrin, alpha 1 [ Rattus norvegicus ]
• Official Symbol: ITGA1
• Synonyms: ITGA1; integrin, alpha 1; integrin alpha-1; VLA-1; laminin and collagen receptor; CD49 antigen-like family member A
• Gene ID: 25118
• mRNA Refseq: NM_030994
• Protein Refseq: NP_112256