Active Recombinant Mouse ITGA1&ITGB1 protein, His-tagged

• Cat.No.: ITGA1&ITGB1-0310M
• Product Overview: Recombinant Mouse ITGA1&ITGB1 protein(Phe29-Pro1141(ITGA1)&Gln21-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Collagen IV at 2 μg/mL (100 μL/well) can bind Mouse ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 10-78 ng/mL.
• Molecular Mass: The reducing (R) protein migrates as 150-200 kDa (ITGA1) and 90-130 kDa (ITGB1) respectively due to glycosylation.
• Proteinlength: Phe29-Pro1141(ITGA1)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Itga1 integrin alpha 1 [ Mus musculus ]
• Official Symbol: ITGA1
• Synonyms: ITGA1; integrin alpha 1; integrin alpha-1; VLA-1; laminin and collagen receptor; CD49 antigen-like family member A; Vla1; CD49A; E130012M19Rik
• Gene ID: 109700
• mRNA Refseq: NM_001033228
• Protein Refseq: NP_001028400