Active Recombinant Human ITGA11 & ITGB1 Heterodimer Protein, His-tagged

• Cat.No.: ITGA11&ITGB1-357H
• Product Overview: Recombinant Human ITGA11 & ITGB1 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Phe 23 - Pro 1141 (ITGA11) & Gln 21-Asp 728 (ITGB1) (Accession # Q9UKX5-1 (L524R) (ITGA11) & P05556-1 (ITGB1)). Predicted N-terminus: Phe 23 (ITGA11) & Gln 21 (ITGB1). Human ITGA11 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA11 and ITGB1, has a calculated MW of 131.6 kDa (ITGA11) & 82.7 kDa (ITGB1). Subunit ITGA11 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 130 kDa (ITGA11) and 100 kDa (ITGB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Phe 23 - Pro 1141 (ITGA11) & Gln 21-Asp 728 (ITGB1)
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Native Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Recombinant Human ITGA11 & ITGB1 Heterodimer Protein, His-tagged with a linear range of 0.156-2.5 μg/mL (QC tested).
• Molecular Mass: 131.6 kDa (ITGA11) & 82.7 kDa (ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA11 integrin, alpha 11 [ Homo sapiens ] & ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: ITGA11 & ITGB1
• Synonyms: ITGA11; integrin, alpha 11; integrin alpha-11; HsT18964; & ITGB1; FNRB, MDF2, MSK12; integrin beta-1; CD29; GPIIA; FNRB; MDF2; VLAB; MSK12; VLA-BETA
• Gene ID: 22801 & 3688
• mRNA Refseq: NM_001004439 & NM_002211
• Protein Refseq: NP_001004439 & NP_002202
• MIM: 604789 & 135630
• UniProt ID: Q9UKX5 & P05556