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Active Recombinant Human ITGAV&ITGB5 protein, His-tagged

Cat.No. : ITGAV&ITGB1-7119H
Product Overview : Recombinant Human ITGAV&ITGB5 protein(Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)), fused with His tag, was expressed in HEK293.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Vitronectin at 2 μg/mL (100 μL/well) can bind Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free with a linear range of 2-20 ng/mL.
Molecular Mass : Human ITGAV&ITGB5 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 112.9 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 85-95 kDa (ITGB5) respectively due to glycosylation.
Protein Length : Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : ITGAV integrin, alpha V [ Homo sapiens ]
Official Symbol : ITGAV
Synonyms : ITGAV; integrin, alpha V; antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR; integrin alpha-V; CD51; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; VNRA; VTNR; DKFZp686A08142;
Gene ID : 3685
mRNA Refseq : NM_001144999
Protein Refseq : NP_001138471
MIM : 193210
UniProt ID : P06756

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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