Active Recombinant Human L1CAM Protein, His/Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: L1CAM-526HAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant human L1CAM (Accession # EAW63869) Ile25-Gln1096, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 25-1096 a.a.
• Form: Lyophilized
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.Able to significantly enhance neurite outgrowth when immobilized as a 3 μL droplet containing 100 ng on a nitrocellulose-coated microplate.
• Molecular Mass: Recombinant Human L1CAM, His-tagged has a calculated MW of 121.3 kDa. In SDS-PAGE migrates as 160-180 kDa, reducing conditions.
• N-terminal Sequence Analysis: Ile 25
• Purity: > 95 % by SDS-PAGE under reducing conditions and visualized by silver stain.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: L1CAM L1 cell adhesion molecule [ Homo sapiens ]
• Official Symbol: L1CAM
• Synonyms: L1CAM; L1 cell adhesion molecule; antigen identified by monoclonal R1 , HSAS, HSAS1, MASA, MIC5, S10, SPG1; neural cell adhesion molecule L1; CD171; antigen identified by monoclonal R1; S10; HSAS; MASA; MIC5; SPG1; CAML1; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Gene ID: 3897
• mRNA Refseq: NM_000425
• Protein Refseq: NP_000416
• MIM: 308840
• UniProt ID: P32004