Active Recombinant Human LAMA2 protein, Fc-tagged

• Cat.No.: LAMA2-0367H
• Product Overview: Recombinant Human LAMA2 protein(Leu1900-Ala2722(Lamininalpha2E8)&Leu1573-Gln1798(Lamininbeta2E8)&Asn1364-Pro1609(Laminingamma1E8)), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1900-2722;1573-1798;1364-1609 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 221 E8, Fc Tag with a linear range of 0.2-20 ng/mL.Immobilized Human Laminin 221 E8, Fc Tag at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.02-0.313 μg/mL.
• Molecular Mass: The non-reducing (NR) protein migrates as 118-130 kDa due to glycosylation.
• Proteinlength: Leu1900-Ala2722(Lamininalpha2E8)&Leu1573-Gln1798(Lamininbeta2E8)&Asn1364-Pro1609(Laminingamma1E8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAMA2 laminin, alpha 2 [ Homo sapiens ]
• Official Symbol: LAMA2
• Synonyms: LAMA2; laminin, alpha 2; LAMM; laminin subunit alpha-2; congenital muscular dystrophy; merosin; laminin M chain; merosin heavy chain; laminin-2 subunit alpha; laminin-4 subunit alpha; laminin-12 subunit alpha
• Gene ID: 3908
• mRNA Refseq: NM_000426
• Protein Refseq: NP_000417
• MIM: 156225
• UniProt ID: P24043