| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Fc |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant. |
| Bio-activity : |
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.1-4 ng/mL.Immobilized Human ITGA3&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.1-4 ng/mL. |
| Molecular Mass : |
Human Laminin 511 E8, Fc Tag, produced by co-expression of Laminin alpha5E8, Laminin beta1E8 and Laminin gamma1E8, has a calculated MW of 76.7 kDa, 35.0 kDa and 35.7 kDa. The non-reducing (NR) protein migrates as 40-46 kDa (subunit Laminin beta1E8 and subunit Laminin gamma1E8) and 80 kDa (subunit Laminin alpha5E8) respectively due to glycosylation. |
| Proteinlength : |
Glu2655-Ala3327(Lamininalpha5E8)&Asp1714-Leu1786(Lamininbeta1E8)&Asp1528-Pro1609(Laminingamma1E8) |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
