Active Recombinant Human LAMA5 protein, Fc-tagged

• Cat.No.: LAMA5-0375H
• Product Overview: Recombinant Human LAMA5 protein(Glu2655–Ala3327(Lamininalpha5E8)&Leu1573-Gln1798(Lamininbeta2E8)&Asp1528–Pro1609(Laminingamma1E8)), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1573-1798 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human Laminin 521 E8, Fc Tag at 5 μg/mL (100 μL/well) can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.01-0.156 μg/mL.Immobilized Human Laminin 521 E8, Fc Tag at 5 μg/mL (100 μL/well) can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free with a linear range of 0.02-0.313 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminusThe protein has a calculated MW of 76.7 kDa | 51.3 kDa | 35.6 kDa. The protein migrates as 92-97 kDa and 100-116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu2655–Ala3327(Lamininalpha5E8)&Leu1573-Gln1798(Lamininbeta2E8)&Asp1528–Pro1609(Laminingamma1E8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LAMA5 laminin, alpha 5 [ Homo sapiens ]
• Official Symbol: LAMA5
• Synonyms: LAMA5; laminin, alpha 5; laminin subunit alpha-5; laminin alpha-5 chain; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha; KIAA1907
• Gene ID: 3911
• mRNA Refseq: NM_005560
• Protein Refseq: NP_005551
• MIM: 601033
• UniProt ID: O15230