Active Recombinant Human LGALS3 Protein (Ala2-Ile250), N-His tagged, Animal-free, Carrier-free

• Cat.No.: LGALS3-191H
• Product Overview: Recombinant Human LGALS3 Protein (Ala2-Ile250) with N-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Ala2-Ile250
• Description: Galectin-3 (Gal-3) is one of the lectin family members, which is the only chimera‑type galectin, containing one carbohydrate recognition domain (CRD) connected to a long, flexible N‑terminal domain. The C‑terminal CRD is responsible for β-galactoside binding, and the N‑terminal domain is essential for its multimerization, and interaction with other intracellular proteins. Galectin-3 is predominantly presented in the cytoplasm and expressed on the cell surface, and then often secreted into biological fluids, such as serum and urine. Numerous studies have indicated that galectin-3 plays a crucial role in cell proliferation, apoptosis, gene expression, immune surveillance, inflammation, fibrosis, and host defense.
• Form: Lyophilized
• Bio-activity: Measured by its ability to chemoattract human PBMC using a concentration range of 2.5-25 μg/mL. Note: Results may vary from different PBMC donors.
• Molecular Mass: The protein has a calculated MW of 27 kDa.
  The protein migrates as 32 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: LGALS3 lectin, galactoside-binding, soluble, 3 [ Homo sapiens (human) ]
• Official Symbol: LGALS3
• Synonyms: LGALS3; lectin, galactoside-binding, soluble, 3; LGALS2; galectin-3; galectin 3; GALIG; MAC 2; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; L31; GAL3; MAC2; CBP35; GALBP
• Gene ID: 3958
• mRNA Refseq: NM_001177388
• Protein Refseq: NP_001170859
• MIM: 153619
• UniProt ID: P17931