Active Recombinant Human MDM2, GST-tagged
Cat.No. : | MDM2-2320H |
Product Overview : | Recombinant Human MDM2, fused with an N-terminal GST (glutathione S-transferase) tag, was expressed in E. coli. |
Availability | February 10, 2025 |
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Description : | This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. |
Source : | E. coli |
Species : | Human |
Tag : | GST |
Form : | 50 mM Hepes pH 8, 200 mM NaCl, 10% glycerol, 5 mM TCEP |
Bio-activity : | Recombinant Human GST-Mdm2 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Mdm2 concentration of 0.5-4 µM. |
Molecular Mass : | 82 kDa |
Purity : | >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. |
Storage : | Avoid repeated freeze-thaw cycles.12 months from date of receipt, -70 ºC as supplied.3 months, -70 ºC under sterile conditions after opening. |
Gene Name : | MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase [ Homo sapiens (human) ] |
Official Symbol : | MDM2 |
Synonyms : | MDM2; MDM2 proto-oncogene, E3 ubiquitin protein ligase; HDMX; hdm2; ACTFS; E3 ubiquitin-protein ligase Mdm2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; double minute 2, human homolog of; p53-binding protein; oncoprotein Mdm2 |
Gene ID : | 4193 |
mRNA Refseq : | NM_001145337 |
Protein Refseq : | NP_001138809 |
MIM : | 164785 |
UniProt ID : | Q00987 |
Chromosome Location : | 12q14.3-q15 |
Pathway : | AKT phosphorylates targets in the cytosol; Adaptive Immune System; Cellular Senescence |
Function : | enzyme binding; identical protein binding; ligase activity; p53 binding |
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MDM2-651M | Recombinant Mouse MDM2 Protein (1-489 aa), His-tagged | +Inquiry |
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MDM2-0427H | Recombinant Human MDM2 Protein (S17-N111), His tagged | +Inquiry |
◆ Lysates | ||
MDM2-4404HCL | Recombinant Human MDM2 293 Cell Lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewValidated in multiple assays.
Great for cell culture.
Consistent performance, highly reproducible.
Q&As (7)
Ask a questionMDM2 binds directly to p53, inhibiting its transcriptional activity. Additionally, MDM2 ubiquitinates p53, targeting it for proteasomal degradation, thus regulating p53 levels in the cell.
The MDM2-p53 feedback loop is crucial for cellular homeostasis. When DNA damage occurs, p53 levels rise, leading to cell cycle arrest or apoptosis. Elevated p53 can induce MDM2 expression, which in turn downregulates p53, ensuring that p53 activity is transient.
Amplifications or overexpression of MDM2 can lead to reduced p53 activity, even in the presence of DNA damage or other stress signals, potentially contributing to uncontrolled cell proliferation and tumorigenesis.
Several small molecule inhibitors, like Nutlin-3 and RG7112, target MDM2 to prevent its interaction with p53, thereby stabilizing and activating p53. These inhibitors hold therapeutic potential, especially in tumors with wild-type p53 but overexpressed MDM2.
Besides p53, MDM2 interacts with other proteins, including E2F1 and RB, influencing cell cycle progression and apoptosis. The balance of these interactions can determine cell fate.
The MDM2 gene encodes a nuclear-localized E3 ubiquitin ligase. Its primary function is to regulate the p53 tumor suppressor protein's stability and activity.
Therapeutic strategies targeting MDM2 include the use of MDM2 inhibitors to reactivate p53, combination therapies with DNA-damaging agents, and strategies to disrupt MDM2's interaction with other proteins to modulate cell cycle and apoptosis.
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