Active Recombinant Human MFGE8 protein, His-tagged

• Cat.No.: MFGE8-587H
• Product Overview: Recombinant Human MFGE8 protein(Leu24-Cys387), fused with C-terminal His tag, was expressed in Insect Cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 24-387 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
• Bio-activity: When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 μg/mL, 100 μL/well), 45-85% cells will adhere after 1 hour at 37°C.
• Molecular Mass: 42 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 85 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: MFGE8 milk fat globule-EGF factor 8 protein [ Homo sapiens ]
• Official Symbol: MFGE8
• Synonyms: MFGE8; milk fat globule-EGF factor 8 protein; SPAG10, sperm associated antigen 10; lactadherin; BA46; EDIL1; hP47; HsT19888; MFG E8; OAcGD3S; SED1; sperm surface protein hP47; medin; sperm associated antigen 10; breast epithelial antigen BA46; O-acetyl disialoganglioside synthase; HMFG; MFGM; MFG-E8; SPAG10
• Gene ID: 4240
• mRNA Refseq: NM_001114614
• Protein Refseq: NP_001108086
• MIM: 602281
• UniProt ID: Q08431