Active Recombinant Human/Mouse/Rat/Rhesus/Canine BMP2 protein(Gln283-Arg396), hFc-tagged

• Cat.No.: BMP2-16H
• Product Overview: Recombinant Human / Mouse / Rat / rhesus / Canine BMP2 (NP_001191.1) (Gln 283-Arg 396) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus. The mature form sequences of Human, Mouse, Rat, rhesus and Canine BMP2 are identical.

Specification

• Species: Human/Mouse/Rat/Rhesus/Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Gln283-Arg396
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind recombinant human BMPR-II-Fc in a functional ELISA.
• Molecular Mass: The recombinant human / mouse / Rat / rhesus / Canine BMP2 consists of 351 amino acids and has a predicted molecular mass of 39.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BMP2 bone morphogenetic protein 2 [ Homo sapiens ]
• Official Symbol: BMP2
• Synonyms: BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; bone morphogenetic protein 2A; BDA2
• Gene ID: 650
• mRNA Refseq: NM_001200
• Protein Refseq: NP_001191
• MIM: 112261
• UniProt ID: P12643

Citation

Depot injectable biodegradable nanoparticles loaded with recombinant human bone morphogenetic protein-2: preparation, characterization, and in vivo evaluation

Journal: Drug Design, Development and Therapy    PubMed ID: 26203226    Data: 2022/11/28

Authors: Ali Habiballah Hassan, Khaled Mohamed Hosny, Ghada Naguib

Article Snippet:rhBMP-2 was purchased from Creative BioMart (New York, NY, USA), PLGA Resomer RG 502 H Mw 17 kDa was purchased from Boehringer Ingelheim (Biberach, Germany).. Polycaprolactone (PCL) (molecular weight 45 kDa) and PLGA were purchased from Sigma/Aldrich (St. Louis, Missouri, USA).Polycaprolactone (PCL) (molecular weight 45 kDa) and PLGA were purchased from Sigma/Aldrich (St. Louis, Missouri, USA).

SEM images of rhBMP-2 nanoparticles. Abbreviations: SEM, scanning electron microscopy; rhBMP-2, recombinant human bone morphogenetic protein-2; DDL, Dynamic data links.

Release of rhBMP-2 from nanoparticles prepared with various PLGA:PCL ratios. Abbreviations: rhBMP-2, recombinant human bone morphogenetic protein-2; PLGA, poly lactide- co -glycolide; PCL, polycaprolactone.

Plasma level of BMP-2 after injection of 5 μg/kg of rhBMP-2 nanoparticles into the surgically created alveolar bone defects in the rabbits (group C). Abbreviations: BMP-2, bone morphogenetic protein-2; rhBMP-2, recombinant human BMP-2.