Active Recombinant Human MPL protein, His-tagged

• Cat.No.: MPL-1935H
• Product Overview: Active Recombinant Human MPL protein(P40238-1)(Gln26-Trp491) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 26-491 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human c-MPL, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 67-77 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: MPL myeloproliferative leukemia virus oncogene [ Homo sapiens ]
• Official Symbol: MPL
• Synonyms: MPL; myeloproliferative leukemia virus oncogene; thrombopoietin receptor; CD110; TPOR; TPO-R; proto-oncogene c-Mpl; myeloproliferative leukemia protein; MPLV; C-MPL; THCYT2
• Gene ID: 4352
• mRNA Refseq: NM_005373
• Protein Refseq: NP_005364
• MIM: 159530
• UniProt ID: P40238