Species : |
Cynomolgus |
Source : |
C-His |
Protein Length : |
Gln22-Thr489 |
Tag : |
C-His |
Form : |
Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl, 3 mM DTT, 10% glycerol (pH 7.5). |
Bio-activity : |
Immobilized Cynomolgus c-MPL, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-C-MPL Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. |
Molecular Mass : |
The protein has a predicted MW of 53.48 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
Endotoxin : |
Less than 1EU per μg by the LAL method. |
Purity : |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC. |
Storage : |
Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |