| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
19-1389 aa |
| Conjugation/Label : |
Biotin |
| Description : |
Mrc1 is a conserved checkpoint mediator protein that transduces the replication stress signal to the downstream effector kinase. Mrc1 and its vertebrate homologue Claspin serve as a mediator for replication stress checkpoint signaling, receiving the signal from Mec1/Rad3/ATR sensor kinase and transmitting it to the effector Rad53/Cds1/Chk1 kinase. The loss of mrc1 checkpoint activity results in the aberrant activation of late/dormant origins in the presence of hydroxyurea. Tumor-associated macrophages (TAMs) expressing the multi-ligand endocytic receptor mannose receptor (CD206/MRC1) contribute to tumor immunosuppression, angiogenesis, metastasis, and relapse. |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Specificity : |
Protein Ratio: Passed as determined by the HABA assay/binding ELISA. |
| Bio-activity : |
Immobilized Mannan from Saccharomyces cerevisiae at 20 μg/mL (100 μL/well) can bind Biotinylated Human CD206 Protein, His Tag, primary amine labeling with a linear range of 0.156-2.5 μg/mL. |
| Molecular Mass : |
The protein has a calculated MW of 158.6 kDa. The protein migrates as 170-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Purity : |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
