Recombinant Human MRC1 protein, His-tagged

• Cat.No.: MRC1-30H
• Product Overview: Recombinant Human MRC1 protein()(Leu19-Ala1389) was fused to His-tagged and expressed in HEK293

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Leu19-Ala1389
• Description: Mrc1 is a conserved checkpoint mediator protein that transduces the replication stress signal to the downstream effector kinase. Mrc1 and its vertebrate homologue Claspin serve as a mediator for replication stress checkpoint signaling, receiving the signal from Mec1/Rad3/ATR sensor kinase and transmitting it to the effector Rad53/Cds1/Chk1 kinase. The loss of mrc1 checkpoint activity results in the aberrant activation of late/dormant origins in the presence of hydroxyurea. Tumor-associated macrophages (TAMs) expressing the multi-ligand endocytic receptor mannose receptor (CD206/MRC1) contribute to tumor immunosuppression, angiogenesis, metastasis, and relapse.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: The protein has a calculated MW of 158.6 kDa. The protein migrates as 150-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MRC1
• Official Symbol: MRC1
• Synonyms: MMR, CD206, hMR, MRC1, CLEC13D, CLEC13DL, MRC1L1
• Gene ID: 4360
• mRNA Refseq: NM_002438.4
• Protein Refseq: NP_002429.1
• MIM: 153618
• UniProt ID: P22897