Recombinant Human MRC1 protein, His-tagged
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|Product Overview :||Recombinant Human MRC1 protein()(Leu19-Ala1389) was fused to His-tagged and expressed in HEK293|
|Description :||Mrc1 is a conserved checkpoint mediator protein that transduces the replication stress signal to the downstream effector kinase. Mrc1 and its vertebrate homologue Claspin serve as a mediator for replication stress checkpoint signaling, receiving the signal from Mec1/Rad3/ATR sensor kinase and transmitting it to the effector Rad53/Cds1/Chk1 kinase. The loss of mrc1 checkpoint activity results in the aberrant activation of late/dormant origins in the presence of hydroxyurea. Tumor-associated macrophages (TAMs) expressing the multi-ligand endocytic receptor mannose receptor (CD206/MRC1) contribute to tumor immunosuppression, angiogenesis, metastasis, and relapse.|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Molecular Mass :||The protein has a calculated MW of 158.6 kDa. The protein migrates as 150-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Leu19-Ala1389|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.|
|SDS-PAGE of MRC1-30H :||
|Gene Name :||MRC1|
|Official Symbol :||MRC1|
|Synonyms :||MMR, CD206, hMR, MRC1, CLEC13D, CLEC13DL, MRC1L1|
|Gene ID :||4360|
|mRNA Refseq :||NM_002438.4|
|Protein Refseq :||NP_002429.1|
|UniProt ID :||P22897|
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