Active Recombinant Human NT5E protein, His-tagged

• Cat.No.: NT5E-2681H
• Product Overview: Recombinant Human 5'-Nucleotidase/CD73 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Lys547) of human 5'-Nucleotidase/CD73 (Accession #NP_002517.1) fused with a 6×His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys547
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1.Measured by its binding ability in a functional ELISA. Immobilized Human 5-Nucleotidase/CD73 at 2 μg/mL (100 μL/well) can bind 5-Nucleotidase/CD73 Rabbit pAb with a linear range of 0.16-2.93 ng/mL.
  2.Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >33654 pmol/min/μg.
  3.Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is >25,000 pmol/min/μg.
• Molecular Mass: 60-70 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 95% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
• Official Symbol: NT5E
• Synonyms: NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT
• Gene ID: 4907
• mRNA Refseq: NM_001204813
• Protein Refseq: NP_001191742
• MIM: 129190
• UniProt ID: P21589