Active Recombinant Rat Nt5e protein, His-tagged

• Cat.No.: Nt5e-2210M
• Product Overview: Recombinant Rat Nt5e protein(Q66HL0)(Met1-Lys549), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys549
• Form: Lyophilized from sterile 20mM Tris, 120 mM NaCl, 4 mM CaCl2, 20 % glycerol, pH 7.5.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is >15,000 pmol/min/µg.
• Molecular Mass: The recombinant rat NT5E comprises 532 amino acids and predicts a molecular mass of 59.4 kDa. The apparent molecular mass of the recombinant protein is approximately 59 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Nt5e 5 nucleotidase, ecto [ Rattus norvegicus ]
• Official Symbol: Nt5e
• Synonyms: NT5E; 5 nucleotidase, ecto; 5-nucleotidase; 5-NT; ecto-5-nucleotidase; Nt5; CD73; MGC112615
• Gene ID: 58813
• mRNA Refseq: NM_021576
• Protein Refseq: NP_067587