Species : |
Human |
Source : |
Human Cells |
Description : |
PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFaa, ab and bb are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. The PDGFs play an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. They are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. |
Bio-activity : |
The activity was measured by its ability to stimulate the proliferation of 3T3 cells. |
Molecular Mass : |
35, 40, 45 kDa |
Endotoxin : |
<0.5 EU/ μg |
Purity : |
High Purity, >95% by SDS-PAGE |
Stability : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -80 centigrade under sterile conditions after reconstitution. |
Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution in PBS without carrier protein |
Reconstitution : |
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |