Active Recombinant Human PTPN11 protein, GST-tagged

• Cat.No.: PTPN11-31402TH
• Product Overview: Recombinant Human PTPN11(224–529 aa) fused with GST tag at N-terminal was expressed in E. coli.

Specification

• Description: Mammalian PTPases can be subdivided into 1 of 2 broad categories: transmembrane receptor PTPases and intracellular PTPases. PTPN11 is one of the 2 closely related mammalian intracellular PTPases whose sequences encode 2 tandem SRC homology 2 (SH2) domains that are located at the amino-terminal side of a single PTPase catalytic domain. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Bio-activity: 10 U/µg
• Molecular Mass: 62 kDa
• AA Sequence: MSPILGYWKIKGLVQPTRLLLEYLE EKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYI ADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSK LPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPK LVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLVPRG SASAEIESRVRELSKLAETTDKVKQ GFWEEFETLQQQECKLLYSRKEGQR QENKNKNRYKNILPFDHTRVVLHDG DPNEPVSDYINANIIMPEFETKCNN SKPKKSYIATQGCLQNTVNDFWRMV FQENSRVIVMTTKEVERGKSKCVKY WPDEYALKEYGVMRVRNVKESAAHD YTLRELKLSKVGQGNTERTVWQYHF RTWPDHGVPSDPGGVLDFLEEVHHK QESIMDAGPVVVHCSAGIGRTGTFI VIDILIDIIREKGVDCDIDVPKTIQ MVRSQRSGMVQTEAQYRFIYMAVQH YIETLQRRILE
• Purity: > 90%
• Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 centigrade.
• Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
• Storage: >12 months at -80 centigrade. Avoid freeze/thaw cycles.
• Concentration: 0.73 mg/ml
• Publication:
  Serendipitous discovery of light-induced (In Situ) formation of an Azo-bridged dimeric sulfonated naphthol as a potent PTP1B inhibitor (2017)

Gene Information

• Gene Name: PTPN11 protein tyrosine phosphatase, non-receptor type 11 [ Homo sapiens ]
• Official Symbol: PTPN11
• Synonyms: PTPN11; protein tyrosine phosphatase, non-receptor type 11; Noonan syndrome 1 , NS1; tyrosine-protein phosphatase non-receptor type 11; BPTP3; PTP2C; SH PTP2; SHP 2; SHP2; PTP-2C; protein tyrosine phosphatase-2; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C; CFC; NS1; PTP-1D; SH-PTP2; SH-PTP3; MGC14433
• Gene ID: 5781
• mRNA Refseq: NM_002834
• Protein Refseq: NP_002825
• MIM: 176876
• UniProt ID: Q06124
• Chromosome Location: 12q24.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Axon guidance, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; CTLA4 inhibitory signaling, organism-specific biosystem
• Function: hydrolase activity; non-membrane spanning protein tyrosine phosphatase activity; non-membrane spanning protein tyrosine phosphatase activity; protein binding; protein tyrosine phosphatase activity