Active Recombinant Human PVRL3 protein(Met1-Asp400), His-tagged

• Cat.No.: PVRL3-3275H
• Product Overview: Recombinant Human PVRL3 (NP_056295.1) (Met 1-Asp 400) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Asp400
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Human Nectin 3 His at 2 μg/mL (100 μL/well) can bind Human PVRL1/NECTIN1 hFc, the EC50 of Human PVRL1/NECTIN1 hFc is 3-24 ng/mL.
• Molecular Mass: The secreted recombinant human PVRL3 comprises 354 amino acids and has a predicted molecular mass of 39.3 kDa. As a result of glycosylation, rhPVRL3 migrates as an approximately 65-75 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVRL3 poliovirus receptor-related 3 [ Homo sapiens ]
• Official Symbol: PVRL3
• Synonyms: PVRL3; poliovirus receptor-related 3; poliovirus receptor-related protein 3; CD113; CDw113; DKFZP566B0846; nectin 3; PPR3; PVRR3; PRR3; CDW113; NECTIN-3; FLJ90624; DKFZp566B0846
• Gene ID: 25945
• mRNA Refseq: NM_001243286
• Protein Refseq: NP_001230215
• MIM: 607147
• UniProt ID: Q9NQS3