Active Recombinant Human RSPO3 protein, His-tagged

• Cat.No.: RSPO3-3883H
• Product Overview: Active Recombinant Human RSPO3 protein(Q9BXY4-1)(Gln22-Val146) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 22-146 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human R-Spondin 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 14.95 kDa. Due to glycosylation, the protein migrates to 20-25 kDa and 26-32 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_116173.2
• Unit ID: Q9BXY4
• Unit ID-Weblink: https://www.uniprot.org/uniprotkb/Q9BXY4

Gene Information

• Gene Name: RSPO3 R-spondin 3 [ Homo sapiens ]
• Official Symbol: RSPO3
• Synonyms: RSPO3; R-spondin 3; R spondin 3 homolog (Xenopus laevis), thrombospondin, type I, domain containing 2, THSD2; R-spondin-3; FLJ14440; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1
• Gene ID: 84870
• mRNA Refseq: NM_032784
• protein Refseq: NP_116173
• MIM: 610574