Recombinant Human RSPO3 protein, His & T7-tagged

• Cat.No.: RSPO3-1946H
• Product Overview: Recombinant Human RSPO3 aa. (Gln22~His272 (Accession # Q9BXY4)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 32.0kDa
• Protein length: Gln22~His272 (Accession # Q9BXY4)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 9.5

Gene Information

• Gene Name: RSPO3 R-spondin 3 [ Homo sapiens (human) ]
• Official Symbol: RSPO3
• Synonyms: RSPO3; R-spondin 3; PWTSR; THSD2; CRISTIN1; R-spondin-3; R-spondin 3 homolog; protein with TSP type-1 repeat; roof plate-specific spondin-3; thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2
• Gene ID: 84870
• mRNA Refseq: NM_032784.4
• Protein Refseq: NP_116173.2
• UniProt ID: Q9BXY4