Active Recombinant Human SDC1 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: SDC1-0695H
• Product Overview: Biotinylated Recombinant Human SDC1 protein(Gln23-Gly254), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 23-254 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated Human Syndecan-1, Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin (0.5 μg/well) plate. can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.2-6 ng/mL. Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Syndecan-1, Fc,Avitag with a linear range of 0.5-8 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 52.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
• Proteinlength: Gln23-Gly254
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: SDC1 syndecan 1 [ Homo sapiens ]
• Official Symbol: SDC1
• Synonyms: SDC1; syndecan 1; SDC; syndecan-1; CD138; SYND1; syndecan; syndecan proteoglycan 1; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor
• Gene ID: 6382
• mRNA Refseq: NM_001006946
• Protein Refseq: NP_001006947
• MIM: 186355
• UniProt ID: P18827