Recombinant Human SDC1 cell lysate

Cat.No. : SDC1-2253HCL
Product Overview : Human SDC1 / CD138 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
  • Download
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : SDC1 syndecan 1 [ Homo sapiens ]
Official Symbol : SDC1
Synonyms : SDC1; syndecan 1; SDC; syndecan-1; CD138; SYND1; syndecan; syndecan proteoglycan 1; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor;
Gene ID : 6382
mRNA Refseq : NM_001006946
Protein Refseq : NP_001006947
MIM : 186355
UniProt ID : P18827
Chromosome Location : 2p24.1
Pathway : Arf6 trafficking events, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Integrins in angiogenesis, organism-specific biosystem;
Function : cytoskeletal protein binding; glycoprotein binding; lipoprotein lipase activity; protein C-terminus binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (7)

Ask a question
In which tissues is SDC1 mainly expressed? 05/14/2022

SDC1 is predominantly expressed in epithelial tissues.

How is SDC1 linked to the ZEB1 transcription factor? 05/04/2022

The ZEB1 transcription factor acts on SDC1 and represses its expression.

How does SDC1 relate to the expression of the tight junction protein E-cadherin? 03/05/2022

SDC1 protein expression was positively correlated with E-cadherin protein expression and acted synergistically.

What are the effects of inhibiting SDC1 expression? 11/23/2021

Inhibition of SDC1 expression promotes tumor growth, invasion and metastasis.

What diseases is SDC1 involved in? 09/17/2021

SDC1 is involved in the study of diseases such as liver cancer, colon cancer and breast cancer.

Where is SDC1 located? 03/31/2021

SDC1 is localized to the cell membrane.

What biological processes is SDC1 involved in? 11/20/2020

SDC1 is involved in biological processes such as cell proliferation, differentiation, and adhesion.

Customer Reviews (3)

Write a review
Reviews
10/14/2023

    Each customer has a dedicated customer service to provide one-on-one, specialized service that solved the problem in my experiment.

    07/22/2023

      High quality product at a good price, a satisfactory shopping experience.

      07/13/2023

        The product brochure is very detailed and has comprehensive basic information about the SDC1 protein.

        Ask a Question for All SDC1 Products

        Required fields are marked with *

        My Review for All SDC1 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /
        • Service lnquiry:

        Stay Updated on the Latest Bioscience Trends