Recombinant Mouse SDC1 cell lysate

• Cat.No.: SDC1-2089MCL
• Product Overview: Mouse SDC1 / Syndecan-1 / SYND1 / CD138 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Sdc1 syndecan 1 [ Mus musculus ]
• Official Symbol: SDC1
• Synonyms: SDC1; syndecan 1; syndecan-1; synstatin; Sstn; Synd; CD138; Synd1; syn-1; AA408134; AA409076
• Gene ID: 20969
• mRNA Refseq: NM_011519
• Protein Refseq: NP_035649
• Pathway: Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Chylomicron-mediated lipid transport, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem
• Function: cytoskeletal protein binding; glycoprotein binding; protein C-terminus binding

Reviews

10/14/2023

Each customer has a dedicated customer service to provide one-on-one, specialized service that solved the problem in my experiment.

07/22/2023

High quality product at a good price, a satisfactory shopping experience.

07/13/2023

The product brochure is very detailed and has comprehensive basic information about the SDC1 protein.

Q&As

In which tissues is SDC1 mainly expressed?

SDC1 is predominantly expressed in epithelial tissues.

How is SDC1 linked to the ZEB1 transcription factor?

The ZEB1 transcription factor acts on SDC1 and represses its expression.

How does SDC1 relate to the expression of the tight junction protein E-cadherin?

SDC1 protein expression was positively correlated with E-cadherin protein expression and acted synergistically.

What are the effects of inhibiting SDC1 expression?

Inhibition of SDC1 expression promotes tumor growth, invasion and metastasis.

What diseases is SDC1 involved in?

SDC1 is involved in the study of diseases such as liver cancer, colon cancer and breast cancer.

Where is SDC1 located?

SDC1 is localized to the cell membrane.

What biological processes is SDC1 involved in?

SDC1 is involved in biological processes such as cell proliferation, differentiation, and adhesion.