Active Recombinant Human SOD1 Protein, Animal Free, Beta-lactam Antibiotics Free

• Cat.No.: SOD1-1570H
• Product Overview: Active Recombinant Human SOD1 Protein (Animal Free, Beta-lactam Antibiotics Free, low endotoxin level) without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E. coli
• Tag: Non
• Protein Length: 1-154 aa
• Description: The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
• pI: 4.95
• AASequence: MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
• Molecular Mass: 16 kDa
• Bio-activity: 35,380 units/mg
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: ≥ 95% by SDS-PAGE
• Unit Definition: One unit of SOD is defined as the amount of enzyme causing half the maximum inhibition of the oxidation of 7.5 mM NADH in the presence of EDTA, manganese ions, and mercaptoethanol at 23 centigrade and pH 7.4 in lnvitrogen Superoxide Dismutase (SOD) Colorimetric Activity Kit.
• Storage: Store it at -20 centigrade - -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Stable for at least 1 year from receipt of products under proper storage and handling conditions
• Storage Buffer: Sterile 20mM PB (pH 7.0), 0.1M NaCl
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Publications:
  Fabrication, assessment, and optimization of alendronate sodium nanoemulsion-based injectable in-situ gel formulation for management of osteoporosis (2023)

Gene Information

• Gene Name: SOD1 superoxide dismutase 1 [ Homo sapiens (human) ]
• Official Symbol: SOD1
• Synonyms: SOD1; superoxide dismutase 1, soluble; ALS, ALS1, amyotrophic lateral sclerosis 1 (adult); superoxide dismutase [Cu-Zn]; IPOA; SOD, soluble; indophenoloxidase A; Cu/Zn superoxide dismutase; superoxide dismutase, cystolic; ALS; SOD; ALS1; hSod1; homodimer
• Gene ID: 6647
• mRNA Refseq: NM_000454
• Protein Refseq: NP_000445
• MIM: 147450
• UniProt ID: P00441