Active Recombinant Human TIGIT protein, His & Avi-tagged, Biotinylated

• Cat.No.: TIGIT-360H
• Product Overview: Biotinylated Human TIGIT, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # AAI01290). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Description: T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
• Source: HEK293
• Species: Human
• Tag: His & Avi
• Predicted N Terminal: Met 22
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
• Bio-activity: (1) Immobilized Human CD155, Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 0.08-0.31 μg/mL. (2) Immobilized Human CD112, Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 0.6-5 μg/mL.
• Molecular Mass: This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 16.8 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Met 22 - Pro 141
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Storage buffer: 50 mM tris, 100 mM glycine, pH7.5
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: TIGIT T-cell immunoreceptor with Ig and ITIM domains [ Homo sapiens (human) ]
• Official Symbol: TIGIT
• Synonyms: VSIG9; VSTM3; WUCAM; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; Washington University cell adhesion molecule
• Gene ID: 201633
• mRNA Refseq: NM_173799.3
• Protein Refseq: NP_776160.2
• UniProt ID: Q495A1