Active Recombinant Human TIGIT protein, mFc-Avi-tagged, Biotinylated

• Cat.No.: TIGIT-0800H
• Product Overview: Biotinylated Recombinant Human TIGIT protein(Met22-Pro141), fused with mFc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&mFc
• Protein Length: 22-141 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CD155, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, Mouse IgG2a Fc,Avitag with a linear range of 1-10 ng/mL. Immobilized Biotinylated Human TIGIT, Mouse IgG2a Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human TIGIT Antibody with a linear range of 2-16 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag).The protein has a calculated MW of 41.6 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Met22-Pro141
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: TIGIT T cell immunoreceptor with Ig and ITIM domains [ Homo sapiens ]
• Official Symbol: TIGIT
• Synonyms: TIGIT; T cell immunoreceptor with Ig and ITIM domains; V set and immunoglobulin domain containing 9 , V set and transmembrane domain containing 3 , VSIG9, VSTM3; T-cell immunoreceptor with Ig and ITIM domains; DKFZp667A205; FLJ39873; V-set and transmembrane domain containing 3; V-set and immunoglobulin domain containing 9; Washington University cell adhesion molecule; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; WUCAM
• Gene ID: 201633
• mRNA Refseq: NM_173799
• Protein Refseq: NP_776160
• MIM: 612859
• UniProt ID: Q495A1