Active Recombinant Human TLR4/LY96 Complex Protein, His-tagged
Cat.No. : | TLR4-296H |
Product Overview : | Recombinant Human TLR4(His19-Glu563)/LY96(Glu17 - Asn160) fused with His tag at C-terminal was expressed in NS0. |
Availability | February 10, 2025 |
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Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Glu24 (TLR4) and Glu17 (MD-2) |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Bio-activity : | Measured by its ability to inhibit LPS-induced TNF-alpha secretion by PMA-differentiated U937 human histiocytic lymphoma cells. Typically 20 µg/mL of rhTLR4/MD-2 will inhibit >60% of the TNF-alpha secretion induced by 10 ng/mL of LPS. |
Molecular Mass : | Predicted Molecular Mass: 70.6 (TLR4), 19.2 (MD-2) kDa; SDS-PAGE: 95 kDa and 34 kDa, reducing conditions. |
Endotoxin : | <1.0 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Storage : | Avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Protein length : | His19-Glu563;Glu17-Asn160 |
Gene Name : | TLR4 toll-like receptor 4 [ Homo sapiens ] |
Official Symbol : | TLR4 |
Synonyms : | TLR4; toll-like receptor 4; CD284; hToll; TLR 4; homolog of Drosophila toll; TOLL; TLR-4; ARMD10; |
Gene ID : | 7099 |
mRNA Refseq : | NM_003266 |
Protein Refseq : | NP_003257 |
UniProt ID : | O00206 |
Chromosome Location : | 9q33.1 |
Pathway : | Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; IKK complex recruitment mediated by RIP1, organism-specific biosystem; |
Function : | lipopolysaccharide binding; lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphatidylinositol 3-kinase binding; protein binding; receptor activity; transmembrane signaling receptor activity; |
Products Types
◆ Recombinant Protein | ||
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TLR4-421G | Recombinant Goat TLR4 Protein, His-tagged | +Inquiry |
TLR4-290H | Active Recombinant Human TLR4 Protein, His-tagged | +Inquiry |
TLR4-141H | Recombinant Human TLR4 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
TLR4-402HCL | Recombinant Human TLR4 cell lysate | +Inquiry |
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Not For Human Consumption!
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Customer Reviews (3)
Write a reviewPrecision in every experiment—this product is a game-changer for us.
Timely delivery is key, and this product arrived on schedule.
Exceptional customer service—knowledgeable staff guided us through the selection process.
Q&As (5)
Ask a questionTLR4 signaling activates NF-κB, a transcription factor, promoting the expression of pro-inflammatory genes involved in immune responses.
Ligand binding induces conformational changes in TLR4, leading to the recruitment of adaptor proteins, such as MyD88, and the activation of downstream signaling cascades.
The TLR4 signaling pathway activates innate immune responses by detecting PAMPs, triggering the release of cytokines, and promoting the elimination of pathogens.
TLR4 engages in crosstalk with other PRRs, forming a network that enhances the sensitivity and specificity of the immune response to diverse pathogens.
TLR4 consists of extracellular leucine-rich repeats (LRRs) for ligand binding, a transmembrane domain, and an intracellular Toll/interleukin-1 receptor (TIR) domain for signaling.
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