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Active Recombinant Human TLR4/LY96 Complex Protein, His-tagged

Cat.No. : TLR4-296H
Product Overview : Recombinant Human TLR4(His19-Glu563)/LY96(Glu17 - Asn160) fused with His tag at C-terminal was expressed in NS0.
Availability February 10, 2025
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Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : Glu24 (TLR4) and Glu17 (MD-2)
Form : Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to inhibit LPS-induced TNF-alpha secretion by PMA-differentiated U937 human histiocytic lymphoma cells. Typically 20 µg/mL of rhTLR4/MD-2 will inhibit >60% of the TNF-alpha secretion induced by 10 ng/mL of LPS.
Molecular Mass : Predicted Molecular Mass: 70.6 (TLR4), 19.2 (MD-2) kDa;
SDS-PAGE: 95 kDa and 34 kDa, reducing conditions.
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage : Avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Protein length : His19-Glu563;Glu17-Asn160
Gene Name : TLR4 toll-like receptor 4 [ Homo sapiens ]
Official Symbol : TLR4
Synonyms : TLR4; toll-like receptor 4; CD284; hToll; TLR 4; homolog of Drosophila toll; TOLL; TLR-4; ARMD10;
Gene ID : 7099
mRNA Refseq : NM_003266
Protein Refseq : NP_003257
UniProt ID : O00206
Chromosome Location : 9q33.1
Pathway : Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; IKK complex recruitment mediated by RIP1, organism-specific biosystem;
Function : lipopolysaccharide binding; lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphatidylinositol 3-kinase binding; protein binding; receptor activity; transmembrane signaling receptor activity;

Not For Human Consumption!

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12/29/2020

    Precision in every experiment—this product is a game-changer for us.

    04/07/2019

      Timely delivery is key, and this product arrived on schedule.

      08/06/2018

        Exceptional customer service—knowledgeable staff guided us through the selection process.

        Q&As (5)

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        How does TLR4 signaling intersect with the nuclear factor-kappa B (NF-κB) pathway? 11/20/2021

        TLR4 signaling activates NF-κB, a transcription factor, promoting the expression of pro-inflammatory genes involved in immune responses.

        Can you explain the molecular events following TLR4 activation upon ligand binding? 08/14/2020

        Ligand binding induces conformational changes in TLR4, leading to the recruitment of adaptor proteins, such as MyD88, and the activation of downstream signaling cascades.

        What is the role of the TLR4 signaling pathway in innate immunity? 02/18/2019

        The TLR4 signaling pathway activates innate immune responses by detecting PAMPs, triggering the release of cytokines, and promoting the elimination of pathogens.

        Can you elaborate on the crosstalk between TLR4 and other pattern recognition receptors (PRRs)? 10/02/2018

        TLR4 engages in crosstalk with other PRRs, forming a network that enhances the sensitivity and specificity of the immune response to diverse pathogens.

        Can you describe the molecular structure of TLR4 and its functional domains? 04/23/2018

        TLR4 consists of extracellular leucine-rich repeats (LRRs) for ligand binding, a transmembrane domain, and an intracellular Toll/interleukin-1 receptor (TIR) domain for signaling.

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