Active Recombinant Human TNFRSF12A Protein, Fc-tagged

• Cat.No.: TNFRSF12A-221H
• Product Overview: Recombinant human TNFRSF12A isoform 1 (Q9NP84-1) extracellular domain (Glu 28-Trp 79) was fused with the Fc region of human IgG1 at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 28-79 a.a.
• Predicted N Terminal: Glu
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Immobilized Cynomolgus mFc-TNFSF12 at 10 μg/ml (100 μl/well) can bind human Fc-TNFRSF12A, The EC50 of human Fc-TNFRSF12A is 0.07-0.15 μg/ml.
• Molecular Mass: The recombinant human TNFRSF12A/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 313 amino acids and has a calculated molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhTNFRSF12A/Fc monomer is approximately 37 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF12A tumor necrosis factor receptor superfamily, member 12A [ Homo sapiens ]
• Official Symbol: TNFRSF12A
• Synonyms: TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; tumor necrosis factor receptor superfamily member 12A; CD266; FN14; TweakR; tweak-receptor; FGF-inducible 14; type I transmembrane protein Fn14; fibroblast growth factor-inducible immediate-early response protein 14; TWEAKR
• Gene ID: 51330
• mRNA Refseq: NM_016639
• Protein Refseq: NP_057723
• MIM: 605914
• UniProt ID: Q9NP84
• Chromosome Location: 16p13.3
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem