Active Recombinant Human TNFRSF13C Protein, LIgG2b Fc-tagged, low endotoxin

• Cat.No.: TNFRSF13C-321H
• Product Overview: Recombinant Human TNFRSF13C Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1). Predicted N-terminus: Ser 7. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 34.4 kDa. The protein migrates as 42-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: lIgG2b
• Protein Length: Ser 7 - Ala 71
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human BAFF, His Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF13C protein, LIgG2b Fc-tagged, low endotoxin with a linear range of 2-63 ng/mL (QC tested).
  Recombinant Human TNFRSF13C protein, LIgG2b Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF, His Tag with a linear range of 0.2-4 ng/mL (Routinely tested).
• Molecular Mass: 34.4 kDa
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF13C tumor necrosis factor receptor superfamily, member 13C [ Homo sapiens ]
• Official Symbol: TNFRSF13C
• Synonyms: TNFRSF13C; CD268; BAFF receptor; BLyS receptor 3; CVID4; BAFF-R; BROMIX; prolixin; MGC138235
• Gene ID: 115650
• mRNA Refseq: NM_052945
• Protein Refseq: NP_443177
• MIM: 606269
• UniProt ID: Q96RJ3