Active Recombinant Human TNFSF10 Protein (169 aa)

• Cat.No.: TNFSF10-302T
• Product Overview: Recombinant human TRAIL/Apo2L (rhTRAIL) produced in E. coli is a single non-glycosylated polypeptide chain containing 169 amino acids. A fully biologically active molecule, rhTRAIL has a molecular mass of 19.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.

Specification

• Description: TRAIL/Apo2L, also known as Tumor Necrosis Factor Super-Family 10 (TNFSF10), is a pleiotropic cytokine thatbelongs to the TNF superfamily. The full length TRAIL expressed in vivo is a Type II transmembrane protein, although the soluble form also exists and functions. TRAIL has four major receptors: two death receptors DR4 and DR5, two decoy receptors DcR1 and DcR2. TRAIL binds to the death receptors, recruits the FAS-associated death domain, activates caspases 8 and 10, and eventually leads to apoptosis. Because of its antitumor potential, TRAIL is actively studied as a therapeutic agent. On the other hand, abnormal expression of TRAIL in small arteries can induce the proliferation of smooth muscle cells, resulting in increasing vascular remodeling and pulmonary arterial hypertension.
• Source: E. coli
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 40 ng/mL, measured by the cell growth inhibitory assay using RPMI-8226 cells, corresponding to a specific activity of > 2.5 × 10^4 units/mg.
• Molecular Mass: 19.6 kDa, observed by reducing SDS-PAGE.
• Protein Length: 169
• AA Sequence: MVRERGPQRVAAHITGTRGRSNTLS SPNSKNEKALGRKINSWESSRSGHS FLSNLHLRNGELVIHEKGFYYIYSQ TYFRFQEEIKENTKNDKQMVQYIYK YTSYPDPILLMKSARNSCWSKDAEY GLYSIYQGGIFELKENDRIFVSVTN EHLIDMDHEASFFGAFLVG
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE and HPLC.
• Storage: Lyophilized recombinant human TRAIL/Apo2L (rhTRAIL) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhTRAIL remains stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ]
• Official Symbol: TNFSF10
• Synonyms: TNFSF10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10; Apo 2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; chemokine tumor necrosis factor ligand superfamily member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; APO2L; Apo-2L
• Gene ID: 8743
• mRNA Refseq: NM_001190942
• Protein Refseq: NP_001177871
• MIM: 603598
• UniProt ID: P50591