Active Recombinant Human TNFSF12

• Cat.No.: TNFSF12-8461H
• Product Overview: The recombinant human TWEAK is a 17.0 kDa protein, comprising the TNF-like extraCellular domain of TWEAK and was expressed in E. coli

Specification

• Description: Human TWEAK is a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. TWEAK, like many other members of the TNF family, is able to induce interleukin-8 synthesis in a number of cell lines.
• Source: E. coli
• Species: Human
• Form: Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 M L-Arg.
• Bio-activity: The ED?? as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml.
• Molecular Mass: 17.0 kDa
• Endotoxin: <0.1>
• Purity: ≥98% by SDS - PAGE; ≥98% by HPLC
• Storage: The lyophilized human TWEAK is best-stored desiccated below 0℃. Reconstituted TWEAK should be stored in working aliquots at -20℃.
• Reconstitution: Reconstitute in 10 mM Sodium Phosphate (pH 7.5) to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers
• Shipping: Blue ice

Gene Information

• Gene Name: TNFSF12 tumor necrosis factor (ligand) superfamily, member 12 [ Homo sapiens ]
• Official Symbol: TNFSF12?
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; APO3L; DR3LG; TWEAK; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; MGC20669; MGC129581
• Gene ID: 8742
• mRNA Refseq: NM_003809
• Protein Refseq: NP_003800
• MIM: 602695
• UniProt ID: O43508
• Chromosome Location: 17p13.1
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Gene Expression, organism-specific biosystem; Regulation of mRNA Stability by Proteins that Bind AU-rich Elements, organism-specific biosystem; Stabilization of mRNA by HuR, organism-specific biosystem
• Function: cytokine activity; cytokine activity; protein binding; receptor binding; tumor necrosis factor receptor binding