Recombinant Human TNFSF12 protein, hFc-tagged

• Cat.No.: TNFSF12-1547H
• Product Overview: Recombinant Human TNFSF12 protein(O43508-1)(Ser43-His249), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: C-hFc
• Protein Length: Ser43-His249
• Tag: C-hFc
• Form: Supplied as 0.22μm filtered solution in PBS, 200mM L-Arginine (pH 7.4).
• Bio-activity: Immobilized Human TNFSF12, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TNFSF12 Antibody, hFc Tag with the EC50 of 0.14μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 49.62 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF12 tumor necrosis factor (ligand) superfamily, member 12 [ Homo sapiens ]
• Official Symbol: TNFSF12
• Synonyms: TNFSF12; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand superfamily member 12; APO3L; DR3LG; TWEAK; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis; MGC20669; MGC129581
• Gene ID: 8742
• mRNA Refseq: NM_003809
• Protein Refseq: NP_003800
• MIM: 602695
• UniProt ID: O43508